Rainville Paul,Lauber Matthew A.,Gethings Lee,Plumb Robert S.,Cosgrave Eoin,Brousmiche Darryl W.
申请号：
US201715721060
公开号：
US2018094293(A1)
申请日:
2017.09.29
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
Provided herein are methodologies where a glycosylated protein or peptide is subjected to peptide bond cleavage to produce a glycan amino acid complex wherein the N-linked or O-linked glycan is attached. A derivatization reagent is then attached to the N terminus of the amino acid to provide a labeled glycan amino acid complex. The labeled glycan amino acid complex is then separated from the matrix via one or more methods including HILIC SPE, and injected directly onto an LC or LC/MS system for analysis, detection and characterization of the glycosylated protein or the peptide.
