A targeting instrument system is provided and methods for positioning an internal fixation prosthesis, such as an intramedullary rod or nail, including a targeting guide device, an internal fixation prosthesis, and a detachable holding device. The holding device may include an exterior grip portion and a clamp portion defining an interior engaging surface for maintaining a locking alignment between the prosthesis and the targeting guide device during the placement of the internal fixation prosthesis into a subject. The holding device may be formed as a monolithic component including at least two flexible, spaced apart arms defining an elongated receiving channel extending from a proximal end configured to releasably engage the prosthesis and a distal end configured to releasably engage the targeting guide device. Alternatively, the holding device may include two pivotally coupled clamp bodies joined by a shaft rod.
