PROBLEM TO BE SOLVED: To provide a bifidobacteria fermentation method and proliferation method that promote the fermentation process of bifidobacteria and improve the number of viable remaining bacteria of bifidobacteria.SOLUTION: The present invention relates to the bifidobacteria fermentation method and proliferation method that, by performing the fermentation treatment with bifidobacteria using a composition containing a pumpkin processed product, it is found that the proliferation of bifidobacteria is substantially promoted, and a fermentation treatment composition is obtained, in which a viable cell count is large, thereby, promoting the fermentation treatment of bifidobacteria and improving the number of viable remaining bacteria of bifidobacteria, wherein the composition containing the pumpkin treated product consists of 30 mass% or more of a pumpkin crushed material or 16 mass% or more of pumpkin juice based on the total amount of the composition.COPYRIGHT: (C)2016,JPO&INPIT【課題】ビフィズス菌の醗酵処理を促進すると共にビフィズス菌の生存残菌数を向上させるビフィズス菌醗酵方法及び増殖方法の提供。【解決手段】カボチャの処理物を含有する組成物を用いてビフィズス菌で醗酵処理することにより、ビフィズス菌の増殖が大幅に促進され、かつ生菌数が多い醗酵処理組成物が得られることを見出し、ビフィズス菌の醗酵処理を促進すると共に、ビフィズス菌の生存残菌数を向上させるビフィズス菌醗酵方法及び増殖方法。前記カボチャ処理物を含有する組成物は、組成物全量に対して30質量%以上のカボチャ破砕物か又は16質量%以上のカボチャ搾汁からなる、ビフィズス菌発酵方法及び増殖方法。【選択図】なし