T.C. TARIM VE ORMAN BAKANLIĞI DOĞU AKDENİZ GEÇİT KUŞAĞI TARIMSAL ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ
发明人:
BULUNUZ PALAZ, Esra
申请号：
TRTR2019/051098
公开号：
WO2020/171786A1
申请日:
2019.12.17
申请国别(地区):
TR
年份:
2020
代理人:
摘要：
This invention about agricultural sciences, biotechnology, tissue culture and nutrient media, and a new nutrient media and method enabling the multiplication of especially tuber plants with tissue culture and ensuring the multiplication of anatolian orchids and its' characteristic is being consisted of process steps of drying the dry and browned capsules containing seeds of anatolian orchid plant species in a penumbra place for a week； obtaining the seeds by shivering and then screening the cracked capsules with a 60-mesh screen (10)； after passing through blender and adding 15 g/l sucrose, 1.5 g/l activated carbon, 50 ml/l freshly squeezed pineapple juice and 100 g/l fresh potato into 120 mg/l NH4NO3, 75 mg/l MgS04.7H20, 80 mg/l KH2PO4, 85 mg/l Ca(N03)2.4H20, 30 mg/l FeS04.4H20 chemicals, maintaining the pH value at 5.7 with 1 N NaOH or 1 N HCI, adding 6.5 g/l agar, sterilizing in autoclave at 121°C under 1.2 atmosphere pressure for 15 minutes and obtaining the nutrition media (20)； after packaging the obtained seeds as 2 mg in roughing filter paper, keeping them in %25 commercial bleach solution with 1-2 drops of tween 20 in it in order to maintain the superficial sterilization of packed seeds (30)； rinsing the seeds 3 times each for 5 minutes with sterile pure water (40)； taking the seeds to pre-drying in petris within sterile cabin with sterile roughing filter papers placed inside petrises and obtaining sterilized seeds (50)； opening the packages containing sterilized seeds with sterile lance and clamp and seeding the seeds in the packages into the nutrition media (60)； taking the anatolian orchid seeds into in vitro culture and incubating them under 40 pE/m^s1 fluorescent white light in 18/6 (lightness/darkness) photoperiod and in a climate room at 22±2 °C (70)； taking values on germination, protochorm and plant growth in seeds of anatolian orchid species (80)； taking obtained protochorms to a new sub culture with same nutrition media in order for them to turn
