PROCKOP, Darwin, J.,KIM, Dong-ki,NISHIDA, Hidetaka,SHETTY, Askok, K.
申请号：
USUS2016/029874
公开号：
WO2016/176500A1
申请日:
2016.04.28
申请国别(地区):
WO
年份:
2016
代理人:
摘要：
Preparations comprising an enriched population of extracellular vesicles (nEVs) having a negatively charged surface, and that are CD81+ and CD9-, are provided. Improved processes and methods for producing an enriched population of nEVs from non-murine cells, especially human origin cells and/or tissues, are disclosed. Therapeutic methods for using the preparations, including for reducing brain inflammation and treatment of various pathologies associated with brain inflammation, such as by intravenous or intranasal administration, are also described. Methods and preparations for reducing brain inflammation associated with traumatic brain injury (TBI) are also disclosed. A method for treating a patient having suffered a mild traumatic injury (mTMI), or concussion, such as a sports-related head injury, is also disclosed. The nEVs are also demonstrated to reduce the expression level of IL-Ιβ in brain tissue of an animal having had traumatic brain injury. Methods for improving cognitive function and performance in animals after a traumatic brain injury is also demonstrated using the preparations of nEVs disclosed herein.Préparations comprenant une population enrichie de vésicules extracellulaires (nEV) ayant une surface chargée négativement, et qui sont CD81+ et CD9-. Linvention concerne également des processus et des procédés améliorés pour produire une population enrichie de cellules nEV à partir de cellules non murines, en particulier de cellules et/ou tissus dorigine humaine. Linvention concerne aussi des procédés thérapeutiques pour utiliser les préparations, comprenant la réduction de linflammation cérébrale et le traitement de diverses pathologies associées à une inflammation cérébrale, comme par administration intraveineuse ou intranasale. Linvention concerne également des procédés et des préparations pour réduire une inflammation cérébrale associée à une lésion cérébrale traumatique (TBI). Linvention concerne également un procédé permettant de traiter un patient
