The present invention relates to a method for removing undifferentiated embryonic stem cells. In particular, the present invention relates to a method for removing undifferentiated embryonic stem cells by using cardiac glycosides (CGs). The present invention also relates to a method for preparing differentiated cells in which undifferentiated embryonic stem cells have been removed and a method for cell therapy by using such differentiated cells.本發明係有關一種移除未分化的胚胎幹細胞之方法。具體而言,本發明係有關一種利用強心苷類(CGs)移除未分化的胚胎幹細胞之方法。本發明亦有關一種製備分化細胞之方法,其中未分化的胚胎幹細胞係經移除,以及有關一種利用此類分化細胞進行細胞療法之方法。