The invention relates to a pharmaceutical formulation comprising a polypeptide selected from a group containing insulin, an insulin metabolite, an insulin analogue, an insulin derivative or combinations thereof and also comprising a surfactant or combinations of several surfactants, optionally a preservative or combinations of several preservatives and optionally an isotonic agent, buffers or additional auxiliary agents or combinations thereof. According to the invention, said pharmaceutical formulation has an acidic pH value.Farmaceutska formulacija, naznačena time, što sadrži:Gly(A21), Arg(B31), Arg(B32)-humani insulin； najmanje jedan surfaktant odabran od polisorbata 20 (Tween ®20) i polisorbata 80 (Tween®80) u koncentraciji od 15-200ug/ml； najmanje jedan konzervans i vodu； pri čemu je pH farmaceutske formulacije u kiseloj oblasti od 3,5 - 4,5.Prijava sadrži još 12 patentnih zahteva.
