ABSTRACT FOR PATENT "EFFECTIVE PHARMACEUTICAL HERBAL COMPOSITION COMPRISING CHLOROXYLON SWIETENIA (Roxb.) DC FOR THE TREATMENT OF HORMONE DEPENDANT BREAST CANCER". Breast cancer is the most frequent cancer worldwide and a second leading cause of cancer related death among females in the world. The global concern of breast cancer exceeds all other cancers and the incidence rates of breast cancer are increasing. In the midst of emerging drug resistance and evolving heterogeneity in breast cancer, the current chemotherapy approaches used for the treatment of hormone dependent breast cancers have limitations owing to severe side effects. A novel Chloroxylon swietenia (Roxb.) DC based formulation comprising of ten solvent extracts (at least seven) of Chloroxylon swietenia dried leaves in petroleum ether, benzene, chloroform, ethyl acetate, acetone, methanol and water are mixed in desired quantity to make l g mixture. The treatment of formulation with hormone dependent MCF-7 (from human origin) breast cancer cells resulted in inhibition of their growth. The formulation is effective in inducing adverse morphological changes, nuclear fragmentation, apoptosis, inhibit the cell migration, colony formation ability and also reduces the tumor volume of MCF-7 breast cancer cells. The formulation has no any in-vivo acute oral toxicity tested as per OECD 423 with LD50 2000 mg/kg.
