A multi-chamber injection and treatment system includes a first chamber, a second chamber, a mixing element, and a needle portion. The first chamber is generally adapted to receive and advance various cells, while the second chamber is adapted to receive and advance adipose tissue, and can blend or reduce the size of the adipose tissue. The mixing element combines the constituents of the chambers and is in communication with the needle portion for driving the cellular mixture into a target tissue site, such as the mid-corpora of the male penis.