The invention provides a fermented Pennisetum extract, which has an advantageous effect in treating and preventing a cancer without toxicity to normal cells. The invention also provides a process of preparing the fermented Pennisetum extract and the application of the fermented Pennisetum extract in the treatment or prevention of a cancer.本發明提供一種經發酵之狼尾草屬(Pennisetum)萃取物,其在治療及預防癌症中具有有利效果且對正常細胞不具有毒性。本發明亦提供一種製備經發酵之狼尾草屬萃取物之方法及經發酵之狼尾草屬萃取物在治療或預防癌症中之應用。