The present disclosure provides a model of human fibrolamellar hepatocellular carcinoma (FL-HCC) cells maintained as a transplantable tumor line in a host and a method to establish a transplantable human FL-HCC tumor line. Methods of ex vivo cultures of the FL-HCC are provided. Methods of diagnosing and treating FL-HCC tumors are also provided.本揭露內容係提供在一宿主中以可移植性腫瘤株形式維持之人類纖維板層肝細胞癌(FL-HCC)細胞的一模式,及提供建立一可移植性人類FL-HCC腫瘤株之一種方法。提供FL-HCC的離體培養方法。亦提供診斷與治療FL-HCC腫瘤之方法。