Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material； a means for calibrating the position of at least one cartridge； and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct； a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter； a computer module providing the series of commands to a bioprinter； and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry.
