Sexually Assault is generally unwitnessed crime. The number of forensic examination concerning rape victims is constantly increasing. During the past ten years there has been a dramatic increase in the use and reliance on forensic DNA analysis. This has been especially true in sexual assault cases and forcible rape cases that routinely consist of evidentiary stains that are a mixture of body fluids from the victim and offender. Current DNA differential analysis techniques permit the separation of the male and female components of these mixed stains. The absence of spermatozoa is not conclusive evidence that an assault did not occur. The absence of semen could mean no ejaculation occurred. More problem come when person is Aspermic means they ejaculate semen only. Aspermia is the complete lack of semen (not to be confused with Azoospermia, the lack of sperm cells in the semen). In Molecular biology Aspermia, put plainly, describes the condition where there are no sperm present in the male semen. You can have semen without sperm, but you cant have sperm without semen. For example, there could have been a significant time delay between the assault and the collection of specimens, Penetration of the victim could have been made by an object other than the penis. The victim could have inadvertently cleaned or washed away the semen or the specimens could have been collected improperly. The presence of semen, with or without spermatozoa, may corroborate the fact that sexual contact did take place. Actually Males with Aspermia are 20 generally able to reach orgasm and ejaculate, however, and are typically, therefore, sexually active. During the process of Penetration in vagina, and ejaculate of (semen) that is released from the penis of Aspermic person. if any Aspermic person involve in sexual offence with complete lack of semen, how will you count or trace that type of identity. So this type of technique is very useful in Rape case where no Sperm find on victi