Ernie De Boer;Roger Kingdon Craig;Richard Wilhelm Janssens;Dubravka Drabek;Tao Chen;Franklin Gerardus Grosveld
发明人:
Roger Kingdon Craig,Franklin Gerardus Grosveld,Richard Wilhelm Janssens,Dubravka Drabek,Tao Chen,Ernie De Boer
申请号：
US13815812
公开号：
US09365655B2
申请日:
2013.03.15
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The present invention provides a high affinity, antigen-specific, soluble heavy chain-only antibody which: lacks hallmark camelid-related amino acid substitutions and has FR2 substitutions which are not found in antibodies which comprise heavy and light chain； shows increased net hydrophobicity within CDR1 and an increased number of charged amino acids present in CDR3； and comprises one or more amino acid substitutions within the framework β-pleated sheet leading to increased net hydrophobicity within FR1 and increased number of charged amino acids present in FR3. Also provided are VH domains having the same properties, gene segments for their production, methods for their production, transgenic animals and uses of the antibody of the VH domains in therapy.
