A kind of novel cell type generates that and has both Th1 characteristics and dissolved cell activity. It from peripheral blood and is operated to Th1 characteristics after these TH1/limiter unit CD4+ lymphocyte depletions, such as production IFN-gamma can be with the cytotoxic T-Lymphocyte (CTL) that is similar to dissolved cell activity. The CTL activity is using sick cell as target, not normal cell. TH1/killer cell line B-Perforin mechanisms of dissolved cell activity are dead by Telomerase mediated apoptosis and led to the cell of illness. It includes isolation CD4+ cells from peripheral blood that method, which produces and use these TH1/limiter units, and to form TH1/limiter cell, to patient, wherein TH1/killer cell line patient is allogeneic to T cell activation CD4+ with cell of these TH1/limiters with dissolved cell activity is applied.
