E. I. DU PONT DE NEMOURS AND COMPANY;PIONEER HI-BRED INTERNATIONAL, INC.
发明人:
DJUKANOVIC, VESNA,JONES, SPENCER CHARLES,LIU, ZHAN-BIN,LASSNER, MICHAEL,LYZNIK, LESZEK ALEKSANDER
申请号：
CA2954626
公开号：
CA2954626A1
申请日:
2015.07.01
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
