Performance of enhanced visually directed procedures under low ambient lighting conditions. An imaging system includes a first high resolution photosensor configured to acquire at least one first real-time high resolution video signal representing at least one view of the eye in at least one wavelength of light outside of the wavelengths of visible light. A second high resolution photosensor is configured to acquire at least one second real-time high resolution video signal representing the at least one view of the eye inside of the wavelengths of visible light. A control unit is configured to acquire the at least one first real-time high resolution video signal and the at least one second real-time high resolution video signal after the light conditions are low enough such that a pupil of the eye does not constrict substantially from its maximum pupillary diameter, via the first and the second high resolution photosensor, respectively.