NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT;SENJU PHARMACEUTICAL CO., LTD.
发明人:
Noriyuki Azuma,Taku Tanaka,Tadashi Yokoi
申请号：
US15502321
公开号：
US20170191033A1
申请日:
2015.08.07
申请国别(地区):
US
年份:
2017
代理人:
摘要：
An object of the present invention is to provide a method of inducing pluripotent stem cells to differentiate into clinically applicable retinal ganglion cells.The present invention provides a method for inducing pluripotent stem cells to differentiate into retinal ganglion cells that can be used for clinical application. Such method is a method for producing retinal ganglion cells with elongated axons comprising the following steps: (a) a step of inducing pluripotent stem cells to differentiate into retinal progenitor cells via floating culture； (b) a step of inducing the retinal progenitor cells obtained in step (a) to differentiate into retinal ganglion cells via floating culture； and (c) a step of allowing axons to elongate via adhesion culture of the retinal ganglion cells obtained in step (b).
