Maria Chiara Bonini,Pietro Genovese,Philip D. Gregory,Michael C. Holmes,Luigi Naldini,David Paschon,Elena Provasi,Lei Zhang
申请号：
US14591625
公开号：
US20150164954A1
申请日:
2015.01.07
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
