Kenneth H. Grabstein,Michael Van Brunt,Marcello Marelli,William Brady,Jeffrey C. Johnson
申请号：
US14430542
公开号：
US09732367B2
申请日:
2013.09.24
申请国别(地区):
US
年份:
2017
代理人:
摘要：
There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.
