Disclosed is a method of controlling insects, comprising applying at least one spinosyn compound to a locus of a neonicotinoid-resistant insect, wherein the neonicotinoid-resistant insect is in the order of Lepidoptera, Diptera, Homoptera, Thysanoptera, Anoplura, Dermaptera, Blattaria, Hemiptera, Hymenoptera, Isoptera, Mallophaga, Siphonaptera, Thysanura, Acarina, Symphyla, Nematoda, or Orthoptera. also disclosed are methods of applying at least one spinosyn compound to a locus of Drosophila melanogaster having at least one lesion in at least one of Dα1 and Dß2, or having a mutation in the Dß2 subunit of the nicotinic acetylcholine receptor.
