The present invention relates to the production of modified influenza viral proteins in plants. More specifically, the present invention relates to producing and increasing influenza virus-like particle (VLP) production in plants, wherein the VLPs comprise the modified influenza viral proteins, such as modified influenza hemagglutinin (HA). The HA protein may comprising an amino acid sequence comprising at least one substitution when compared to a corresponding wildtype amino acid sequence. Further provided are nucleic acid encoding the modified HA protein. Furthermore methods of producing an influenza virus like particle (VLP) and methods of increasing yield of production of an influenza virus like particle (VLP) in a plant, portion of a plant, or a plant cell, are also provided.本發明涉及在植物中經修飾的流感病毒蛋白質的生產。更具體地，本發明涉及在植物中生產和增加流感類病毒顆粒(VLP)的生產，其中VLP包含經修飾的流感病毒蛋白質，例如經修飾的流感血球凝集素(HA)。當與相應的野生型胺基酸序列比較時，該HA蛋白質可包含含有至少一個取代的胺基酸序列。還提供了編碼經修飾的HA蛋白質的核酸。此外，還提供了生產流感類病毒顆粒(VLP)的方法和增加植物、植物的一部分或植物細胞中流感類病毒顆粒(VLP)的生產的產量的方法。
