PROCÉDÉ DE PRÉPARATION D'UNE SOLUTION DE PROTÉINE DE SOIE FLUORESCENTE EXTRAITE À PARTIR DE COCONS TRANSGÉNIQUES ET PROCÉDÉ DE FABRICATION D'ÉCHAFAUDAGE À L'AIDE D'UNE TELLE SOLUTION
PARK, Chan Hum,박찬흠,JEONG, Ju Yeon,정주연,JU, Hyung Woo,주형우,KIM, Soo Hyeon,김수현,KIM, Dong Wook,김동욱,MOON, Bo Mi,문보미,PARK, Hyun Jung,박현정,PARK, Ye Ri,박예리,LEE, Min Chae,이민채,LEE, Ok Joo,이옥주,LEE, Jung Min,이정민
申请号：
KRKR2016/006149
公开号：
WO2017/030277A1
申请日:
2016.06.10
申请国别(地区):
KR
年份:
2017
代理人:
摘要：
The present invention provides a method for preparing a fluorescent silk fibroin solution, the method comprising the steps of: a) putting fluorescent cocoons in an aqueous solution containing a refining agent, followed by heating at 40-60℃ for 8-24 h and then washing with distilled water, thereby obtaining refined fluorescent silk fibroin； b) dissolving the refined fluorescent silk fibroin in a solvent, in which 15 mg to 1.5 g of dithiothreitol (DTT) is mixed with each 100 ㎖ of 9-9.6 M LiBr, at 40-60℃ for 1-5 h； and c) dialyzing the dissolved fluorescent silk fibroin in primary distilled water for 48-96 h. The refining agent preferably contains alcalase and sodium bicarbonate (NaHCO3). An existing method for preparing a silk fibroin solution from a fluorescent cocoon has disadvantages in that silk fibroin is not dissolved at a low temperature and the fluorescence is lost. However, according to the present invention, a method for adding a reductant has advantages in that a fluorescent silk fibroin solution can be prepared at a low temperature and the fluorescence is maintained. Therefore, according to the present invention, the fluorescent silk fibroin can be mass-produced and a biocompatible fluorescent protein can be easily prepared and provided, and thus, materials can be provided that are applicable to bio-industries, such as scaffolds for tissue regeneration, bio-imaging, and biosensors through and biochips.La présente invention concerne un procédé de préparation d'une solution de fibroïne de soie fluorescente, le procédé comprenant les étapes consistant : a) à placer les cocons fluorescents dans une solution aqueuse contenant un agent de purification, puis à chauffer de 40 à 60 °C pendant 8 à 24 h, à laver ensuite au moyen d'eau distillée, pour obtenir ainsi de la fibroïne de soie fluorescente purifiée ； b) à dissoudre la fibroïne de soie fluorescente purifiée dans un solvant, dans lequel de 15 mg à 1,5 g de dithiothréitol (DTT) sont mélangés tous les 100 ㎖ de
