The present invention provides a method for detecting an MG53 related disease or predicting a risk of an MG53 related disease, comprising the following steps: a) obtaining a test sample； and b) detecting cell-free MG53 in the test sample. The present invention also provides a method for reducing or inhibiting activity of cell-free MG53 in a subject, comprising administrating an effective amount of MG53 inhibitor to a subject in need thereof. Also provided are an MG53 antibody, a nucleic acid encoding the antibody, a clone or expression vector including the nucleic acid, a host cell including the clone or expression vector, and a pharmaceutical composition including above.本申請案關於一種用於檢測MG53相關疾病或預測MG53相關疾病的風險的方法，該方法包括以下步驟：a.獲得待測樣品；和b.檢測在所述待測樣品中的細胞外游離MG53。本申請案還關於一種用於降低或抑制個體中細胞外游離MG53活性的方法，該方法包括向需要其的個體施用有效量的MG53抑制劑。此外，本申請案還關於一種MG53抗體或其抗原結合片段，編碼該等抗體或其抗原結合片段的核酸，包括該等核酸的殖株或表達載體，包括該等殖株或表達載體的宿主細胞，以及包括上述各產品的藥物組合物。再者，本申請案還關於一種用於檢測細胞外游離MG53的檢測試劑及其用途以及MG53抑制劑在製備用於降低或抑制個體中細胞外游離MG53的藥物中的用途。
