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СТАБИЛИЗИРОВАННОЕ СОЕДИНЕНИЕ ГОРМОНА РОСТА
专利权人:
НОВО НОРДИСК ХЕЛС КЕА АГ (CH)
发明人:
ДЕМЮТ Хелле (DK),СЬЁДТ Кристине Бруун Сьёдт (DK),НЁРСКОВ-ЛАУРИТЦЕН Лайф (DK),ЧЕН Дзяньхэ (DK),ТЮГЕСЕН Петер (DK)
申请号:
RU2012134974/10
公开号:
RU2012134974A
申请日:
2011.01.24
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A compound of growth hormone, comprising one or more additional disulfide bonds and at least one additional single point mutation as compared with human growth hormone (hGH), having the sequence of SEQ ID NO 1.2. Growth hormone compound according to claim 1, wherein the growth hormone compound comprises one or more additional disulfide bonds linking the loop segment and the helical segment.3. Growth hormone compound according to claim 1, wherein the compound comprises at least one pair of mutations corresponding R16C / L117C, A17S / E174S, N21S / M170S, D26C / V102C, D26C / Y103C, N47C / T50C, Q49C / G161C, F54C / Y143C, F54C / S144C, F54C / F146C, S55C / Y143C, S57C / Y143C, I58C / Q141C, I58C / Y143C, I58C / S144C, P59C / Q137C, R61S / E66S, R61S / T67S, S71C / S132C, L73C / S132C, L73C / F139C, R77C / I138C, R77C / F139C, L81C / Q141C, L81C / Y143C, Q84C / Y143C, Q84C / S144C, S85C / Y143C, S85C / S144C, P89C / F146C, F92C / F146C, F92C / T148C, R94C / D107C, V102C / A105C, L156C / F146C, L156C / T148C and / or V185C / S188C in SEQ ID NO 1.4. Growth hormone compound according to claim 1, wherein the growth hormone compound comprises one or more additional disulfide bonds, and wherein at least one of their cysteines present in loop 3 (L3), corresponding AK128-154 consisting of SEQ ID NO 1.5. Growth hormone compound according to claim 4, wherein the growth hormone compound comprises one or more additional disulfide bonds linking L3 with helix 2 (H2) or loop 1 (L1) .6. Growth hormone compound according to claim 3, wherein the compound comprises at least one pair of mutations corresponding N21S / M170, D26C / V102C, D26C / Y103C, F54C / Y143C, F54C / S144C, S55C / Y143C, S57C / Y143C, I58C / Q141C, I58C / Y143C, I58C / S144C, P59C / Q137C, S71C / S132C, L81C / Y143C, Q84C / Y143C, S85C / Y143C, S85C / S144C, F92C / T148C and / or R94C / D107C consisting of SEQ ID NO 1.7. Growth hormone compound according to claim 1, wherein the growth hormone compound has increase
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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