PROBLEM TO BE SOLVED: To provide a method for preparing a longan seed extract, and to provide uses thereof.SOLUTION: A preparation method includes the following steps: (1) formulating an extracting solution with a specific concentration (2) heating the extracting solution to a specific temperature (3) adding powdered longan seeds to the solution and extracting (4) filtering the extract and then concentrating it (5) lyophilizing the concentrated solution and (6) finishing the preparation of a longan seed extract. Test results show that the longan seed extract prepared by the method of this invention produces an anti-inflammatory response, reduces the generation of uric acid, inhibits bacterial activity, improves the growth of skin keratinocytes, and promotes wound healing, without damaging internal organs or increasing the burden of internal organs.