The present invention relates to fusion proteins comprising fragments from toxin A and/or toxin B of Clostridium difficile, in particular the invention relates to proteins comprising a first fragment and a second fragment, wherein [0205] the first fragment is a toxin A repeating domain fragment； [0206] the second fragment is a toxin B repeating domain fragment； [0207] the first fragment has a first proximal end； [0208] the second fragment has a second proximal end； and wherein the first fragment and the second fragment are adjacent to one another and wherein the polypeptide elicits antibodies that neutralize toxin A or toxin B or both. The invention further relates to compositions comprising fragments or variants of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33 or SEQ ID NO:34 or SEQ ID NO:35.
