KAI MASAAKI,甲斐 雅亮,SHIBATA TAKAYUKI,柴田 孝之,KABASHIMA TSUTOMU,椛島 力
申请号：
JP2011045485
公开号：
JP2012179027A
申请日:
2011.03.02
申请国别(地区):
JP
年份:
2012
代理人:
摘要：
PROBLEM TO BE SOLVED: To provide an artificial double-stranded nucleic acid that exhibits an excellent RNA interference effect on a virus such as an HIV being easily mutated and having high diversity, and has high nuclease resistance and intracellular introduction efficiency.SOLUTION: The artificial double-stranded nucleic acid includes: an anti-sense chain nucleic acid of 15-30 base length containing a base sequence complementary to a base sequence of at least 15 continuous bases of a target base sequence composed of a base sequence in which one to several bases are substituted, deleted or added in a specific base sequence and a sense chain nucleic acid of 15-30 base length containing a base sequence complementary to the anti-sense chain nucleic acid in which at least one cytosine in the anti-sense chain or the sense chain is substituted with a bicyclic cytosine derivative.COPYRIGHT: (C)2012,JPO&INPIT【課題】HIVなどの変異しやすく多様性の高いウイルスに対しても優れたRNA干渉効果を発揮し、またヌクレアーゼ耐性及び細胞内導入効率の高い、人工二本鎖核酸の提供。【解決手段】特定の塩基配列において1~数個の塩基が置換、欠失もしくは付加された塩基配列からなる標的塩基配列の少なくとも15塩基の連続する塩基配列に相補的な塩基配列を含む15~30塩基長のアンチセンス鎖核酸と、当該アンチセンス鎖核酸に相補的な塩基配列を含む15~30塩基長のセンス鎖核酸とからなり、かつアンチセンス鎖またはセンス鎖中の少なくとも1個のシトシンを2環状シトシン誘導体に置換した人工二本鎖核酸。【選択図】なし
