Methods of using Bacillus Subtilis probiotic supplementation to promote lower circulating TNF-α in resistance trained males are presented. Methods of administering Bacillus Subtilis probiotic supplementation to an athletic population in conjunction with a sound nutrition and training regimen are presented herein. College athletes typically undergo periods of elevated stress both physically and mentally which may negatively affect recovery and adaptation. 12-weeks of probiotic supplementation resulted in attenuated circulating TNF-α concentrations in college athletes following offseason training.