The device (1) for the preparation of containment units (4) of biological liquids includes a main body (2) which defines at least a filling channel (3), having at least an inlet gap (3a) of a biological liquid and at least an air outlet gap (3b), and includes a plurality of containment units (4) arranged in succession to one another and defining respective containment chambers (5) positioned along the filling channel (3) and placed in between the inlet gap (3a) and the outlet gap (3b).