SOMALOGIC, INC.;SCHNEIDER, DANIEL J.;WILCOX, SHERI K.;ZICHI, DOMINIC;NIEUWLANDT, DAN;CARTER, JEFF;GOLD, LARRY
发明人:
SCHNEIDER, DANIEL J.,WILCOX, SHERI K.,ZICHI, DOMINIC,NIEUWLANDT, DAN,CARTER, JEFF,GOLD, LARRY
申请号：
WO2008US70371
公开号：
WO2009012410(A1)
申请日:
2008.07.17
申请国别(地区):
世界知识产权组织国际局
年份:
2009
代理人:
摘要：
The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.
