Simard, John J.L.,Diamond, David C.,Qui, Zhiyong,Lei, Xiang-Dong
申请号：
AU2008229722
公开号：
AU2008229722B2
申请日:
2008.09.30
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
#$%^&*AU2008229722B220120906.pdf#####ABSTRACT The invention disclosed herein is directed to methods of identifying a polypeptide suitable for epitope liberation including, for example, the steps of identifying an epitope of interest； providing a substrate polypeptide sequence including the epitope, wherein the substrate polypeptide permits processing by a proteasome； contacting the substrate polypeptide with a composition including the proteasome, under conditions that support processing of the substrate polypeptide by the proteasome； and assaying for liberation of the epitope. The invention further relates to vectors including a housekeeping epitope expression cassette. The housekeeping epitope(s) can be derived from a target-associated antigen, and the housekeeping epitope can be liberatable, that is capable of liberation, from a translation product of the cassette by immunoproteasome processing. The invention also relates to a method of activating a T cell comprising contacting a substrate polypeptide with an APC and contacting the APC with a T cell.Figure 1. pMA2M ISS (3) &NIS (7) CMVp (1) pMB ori rev (6) 3315 bp BGH poly A (4) Kan R( Figure Legend: Code in Figure Genetic Element Region 1-M p - Cytomeglovirus Enhancer/Promotor- 63-637 2. SAsjr. -P s . 696-983 3. ISS Immunostimulatory Sequence 3220-3226 4. BGH poly A' Bovine Growth Hormone 1028-1045 Polyadenylation Signal 5. Kan R Kanamycin Resistance Gene 1431-2225 6. pMB ori rev Bacterial pMB Origin of Replication 3165-2492 7. NIS Nuclear Import sequence from 3227-3304 Simian Virus 40-72bp repeat 1/10
