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Fluoroimmunoassay Method
专利权人:
发明人:
Hiroshi Ueda,Ryoji Abe,Masaki Ihara,Hiroaki Takagi
申请号:
US15183974
公开号:
US20160349266A1
申请日:
2016.06.16
申请国别(地区):
US
年份:
2016
代理人:
摘要:
An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase a step (b) of measuring the fluorescence intensity of the fluorescent dye and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye.
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