#$%^&*AU2018205060A120180726.pdf#####ABSTRACT Microparticles are prepared by a method that includes: (a) forming a layer comprising a first polymer on a solid surface by depositing a first composition one or more times on the solid surface, wherein the first composition comprises the first polymer and a first solvent, and evaporating the first solvent in the first composition； (b) forming one or more layers comprising a second polymer and a therapeutic agent by depositing a second composition on all or part of the layer formed in step (a), wherein the second composition comprises the second polymer, the therapeutic agent, and a second solvent； and evaporating the second solvent in the second composition； and (c) forming an additional layer comprising a third polymer by depositing a third composition one or more times on a previously formed layer, wherein the third composition comprises the third polymer and a third solvent； and evaporating the third solvent in the third composition. 600 50 -k AA* 10 . ..... ... .................. ..................................... M 0 ..... ........... . .. . . . . . . . . . . . . . . .. . . . . . . .... . ..... C.. ~.. . . . ........ .............. ...... 0 2 4 6 8 10 12 14 16 1s 20 Weeks Sample Sample Drug Inner Layer Polymer Outer Layer No. Description Load Polymer (% w/w) Sample 1 Brinzolamide 11 9 115 kDa PLGA None suspension in (Evonik Industries/ dicholoromethane Lakeshore Biomaterials 7525 DLG 7E) Sample 2 Brinzolamide 10.2 115 kDa PLGA Two coatings of suspension in (Evonik Industries/ 5% 178 kDa dicholoromethane Lakeshore Biomaterials PLGA (Akina 7525 DLG 7E 8520)
