Cells or tissues are preserved in an unfrozen state in the liquid composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion and the like.
