chimeric polynucleotide, nucleic acid construct, cell, plant, plant explant, transgenic seed, chimeric polypeptide, method of directing a polypeptide of interest to a chloroplast, expression cassette, plant cell, isolated polypeptide农业专利-农业专业知识服务系统

发明人:: DANIEL L. SIEHL,HENRIK ALBERT,JIAN LU,LINDA A. CASTLE,MATTHEW J. HECKERT,YUMIN TAO

摘要：: Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.