It is provided an ultrasonic observation apparatus, an operation method for the ultrasonic observation apparatus, and an operation program for the ultrasonic observation apparatus capable of displaying a vascular channel or noise in tissue of an observation target to be distinguished from another tissue, and capable of clearly displaying the difference in the tissue characterization. A feature amount image is generated on a frequency spectrum approximated by a polynomial such that two spectrum intensities respectively corresponding to two frequencies included in the polynomial are associated with different visual information. Thus, the feature amount image is generated based on information of the frequency spectrum having a characteristic according to the tissue characterization.
