The present invention is to provide a tattoo needle guide tube, which has a tubular body defining a receiving space therein, a first through hole at a top end thereof and a second through hole at a bottom end thereof. Both through holes are in communication with the receiving space, and the outer periphery of the top end can be connected to a main body of an eyebrow tattoo machine, so as to allow a plurality of eyebrow tattoo needles in the main body to pass sequentially through the first through hole and receiving space and have needle tips exposed outside the second through hole. The tattoo needle guide tube is characterized in that the second through hole has a curved shape and is configured and sized to render the needle tips into a curved arrangement, so as for an eyebrow tattooist to make desired hair-like tattoos with ease.
