The present invention relates to an infusion flow regulation device capable of injecting a set flow of an infusion into a body for a predetermined amount of time without being influenced by the infusion amount, which is gradually reduced as injection time passes. The infusion flow regulation device of the present invention comprises: an infusion inflow part (110) having an infusion inlet (111), into which the infusion flows from an infusion bottle, and a vertical center shaft (112) provided at the center thereof an infusion discharge part (120) inserted into and coupled to the lower part of the infusion inflow part through the vertical center shaft, and having an infusion outlet (121), through which the infusion is discharged, formed at one side thereof a flow rate regulating dial (130) inserted and coupled between the infusion inflow part and the infusion discharge part so as to be rotatable in a horizontal state through the vertical center shaft a circular flow path (140) having the vertical center shaft as the center thereof and formed at the top of the flow rate regulating dial a flow rate regulating flow path (150) having the vertical center shaft as the center thereof and formed at the bottom of the flow rate regulating dial, formed in an arc shape having a radius different from that of the circular flow path, and of which a cross sectional area is gradually increased or reduced an upper gasket (160) provided at the bottom of the infusion inflow part so as to come into close contact with the upper part of the flow rate regulating dial a lower gasket (170) provided at the top of the infusion discharge part so as to come into close contact with the lower part of the flow rate regulating dial a first chamber (180) formed to penetrate the upper gasket so as to allow the infusion inlet to communicate with the circular flow path a second chamber (190) formed to penetrate the flow rate regulating dial so as to communicate with the flow rate regulating flow path a con