Federalnoe gosudarstvennoe byudzhetnoe nauchnoe uchrezhdenie Federalnyj nauchnyj tsentr "Vserossijskij nauchno-issledovatelskij i tekhnologicheskij institut ptitsevodstva" Rossijskoj akademii nauk
发明人:
Vertiprakhov Vladimir Georgievich,Grozina Alena Andreevna,Koshcheeva Mariya Valentinovna,Svitkin Valentin Sergeevich
申请号：
RU20170131008
公开号：
RU2670770(C1)
申请日:
2017.09.01
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
SUBSTANCE: invention relates to biochemistry, agriculture and the food industry. Method for determining a trypsin inhibitor in soybeans and their processing products, including the selection and preparation of the analyzed sample, extraction of the soybean inhibitor into the solution, measurement and calculation of trypsin inhibitory activity, differs in that the extraction of the trypsin inhibitor in the test solution is carried out during the homogenization, centrifugation and filtration of the test sample for 10–15 minutes, to determine the activity of trypsin kinetic method, mix soy extract, reagent 1 (buffer pH 8.2) and a control solution of trypsin, incubate for 4 minutes, BAPNA substrate reagent is added, the trypsin inhibitory activity (TIA) is calculated by the formula: TIA = ((TI × 0.025) : 100) × 10000 : 4, where Ti is the amount of trypsin inhibitor, %, calculated by the formula: Ti = ((K – O) : K) × 100 %; K – activity of trypsin in the control sample, units/l; O – activity of tryp
