Emulsion vaccine formulations containing an antigen and an adjuvant in the aqueous phase are used for the vaccination of animals wherein the adjuvant is an acrylic polymer and/or dimethyl dioctadecyl ammonium bromide (DDA). These formulations can be prepared by mixing an aqueous phase containing the antigen and adjuvant with an oil phase in the presence of an emulsifier.本發明係有關一種含有存於水相中之抗原及佐劑的乳劑疫苗調配物,其係用於動物之疫苗接種,其中該佐劑為丙烯酸系聚合物及/或二甲基二(十八烷基)溴化銨(DDA)。該等調配物可藉由在乳化劑存在下將含有抗原及佐劑之水相與油相混合來製備。