A method for producing liposome encapsulating a desired substance is provided. The method is characteristic in that an aqueous phase containing the substance to be encapsulated is added to a homogenous fluid mixture of a phospholipid or a glycolipid and carbon dioxide either under a supercritical condition or under a condition in which temperature or pressure is higher than or equal to its critical point. This method makes it possible to produce liposomes with improved trapping efficiency in fewer steps and without using harmful organic solvents. <；IMAGE>；
