Composition comprises: a detergent component containing sequestering agent, dispersant and wetting agent； and an enzymatic component containing at least one protease, at least one laccase and at least one polysaccharidase. Independent claims are included for: (1) method for the elimination of the biofilms present on a substrate comprising (a) providing a detergent component containing a sequestering agent, dispersant and a wetting agent, and an enzymatic component containing at least one protease, at least one laccase, and at least one polysaccharidase, (b) contacting the detergent component in a solution or dilution in water； (c) contacting the enzymatic component in a solution in a solution formed in the step (b) for forming a solution of the composition； or (b1) contacting the enzymatic component in the solution or dilution in water, and (c1) contacting detergent component in the solution in solution formed in the step (b1) for forming the solution of the composition； and (d) application of the solution of the composition formed in the step (c) or (c1) on the substrate for a period of 15 minutes to 4 hours； and (2) kit comprising at least one sample of a detergent component in solution or in solid form containing a sequestering agent, dispersant and wetting agent and at least one sample component in enzyme solution or in solid form containing at least one protease, at least one laccase and at least one polysaccharidase.
