PROBLEM TO BE SOLVED: To provide a method for efficiently and potently inducing the differentiation of osteoblast cells as compared to the conventional methods.SOLUTION: There is provided a culture substrate for differentiation induction of osteoblast cells which is covered with a fish-derived collagen and has a contact surface to mesenchymal stem cells. There is also provided a method for inducing the differentiation of osteoblast cells which contains a fish-derived collagen contact process in which a cell culture substrate covered with a fish-derived collagen is brought into contact with mesenchymal stem cells, or a method for producing osteoblast cells which contains a fish-derived collagen culture process in which mesenchymal stem cells are cultured by using a cell culture substrate covered with a fish-derived collagen.COPYRIGHT: (C)2012,JPO&INPIT【課題】本発明の課題は、従来法と比較して、効率的且つ強力に骨芽細胞を分化誘導する方法を提供することにある。【解決手段】本発明の課題は、魚類由来コラーゲンが被覆された、間葉系幹細胞との接触面を有する、骨芽細胞分化誘導用培養基材によって解決できる。また、魚類由来コラーゲンで被覆された細胞培養基材と、間葉系幹細胞とを接触させる魚類由来コラーゲン接触工程を含む、骨芽細胞分化誘導方法、又は魚類由来コラーゲンで被覆された細胞培養基材によって、間葉系幹細胞を培養する魚類由来コラーゲン培養工程を含む、骨芽細胞製造方法によって解決できる。【選択図】図1
