Sterilization packaging systems with features for sealing a volume against an ingress of contaminants are provided. Such features include a sealing assembly, where the sealing assembly includes a sheet of sterilization material and a clamp for sealing the sheet of sterilization material against a container to seal the volume of the container from contaminants. Also provided is a sealing assembly including a sheet of sterilization material and a clamp having open position and clamped positions. The clamp is configured to extend about a perimeter of a container, the sheet of sterilization material is disposed between the container and the clamp when the clamp is in the clamped position to seal a volume of the container, and the clamp is held in the clamped position by a latch. Exemplary sterilization packaging systems may include other features as well.
