BACA, MANUEL,PRESTA, LEONARD G.,WELLS, JAMES A.,LOWMAN, LEONARD G.,CHEN, YVONNE MAN-YEE
申请号：
DK07444298
公开号：
DK2301580T3
申请日:
1998.04.03
申请国别(地区):
DK
年份:
2012
代理人:
摘要：
Described herein is a humanized antibody to vascular endothelial growth factor (VEGF). Also described herein is a method for rapidly producing and identifying framework mutations which improve the binding of humanized antibodies to their congnate antigens. In a preferred embodiment, non-human CDRs are grafted onto a human V 1 K I-V H III framework. Random mutagenesis of a small set of critical framework residues is also performed followed by monovalent display of the resultant library of antibody molecules on the surface of filamentous phage. The optimal framework sequences are then identified by affinity-based selection. Optionally, the selected antibodies can be further mutated so as to replace vernier residues which sit at the V L -V H interface by residues which match the non-human parent antibody. The methods described herein can be applied to any non-human antibody. Accordingly, humanized antibodies are provided.
