A kind of closed CO2Blood vessel contrast injection device includes: a pneumatic filter; One first three-way valve,With a first port,One second port and a third port; One second three-way valve,With a first port,One second port and a third port; One first non-return valve; One second non-return valve; One gas-storing bag; An and syringe; Wherein,First non-return valve connects the first port of what first three-way valve,Second non-return valve connects the first port of what second three-way valve,The pneumatic filter is connect with first non-return valve with one first delivery pipe,The gas-storing bag is connect with the second port of first three-way valve with one second delivery pipe,The third port of first three-way valve is connect with second non-return valve with a third delivery pipe,The syringe connects to the second port of second three-way valve.一種密閉式CO2血管顯影注射裝置包含:一氣體過濾器;一第一三向閥,具有一第一端口、一第二端口與一第三端口;一第二三向閥,具有一第一端口、一第二端口與一第三端口;一第一逆止閥;一第二逆止閥;一儲氣袋;以及一針筒;其中,該第一逆止閥連接於該第一三向閥之第一端口,該第二逆止閥連接於該第二三向閥之第一端口,該氣體過濾器與該第一逆止閥以一第一輸送管連接,該儲氣袋與該第一三向閥之第二端口以一第二輸送管連接,該第一三向閥之第三端口與該第二逆止閥以一第三輸送管連接,該針筒係連接至該第二三向閥之第二端口。