A mat for collecting fluid spilled onto a floor that includes a body of small thickness relative to its length and width and having an upper surface defining a plurality of receptacles and a lower surface defining a plurality of diagonally oriented channels or passages that communicate with each other and with the drains. The diagonal channels have distal ends that terminate in an end wall and inner ends that communicate directly or indirectly with a collection channel, the collection channel having a discharge port that is connectable to a suction source. The diagonal channels are formed in two groups that are disposed symmetrically about the collection channel. The mat is especially suitable for use on the floor of an operating room, as well as the floor of a food processing or manufacturing facility where fluids are spilled. The mat may include a anti-microbial, anti-bacterial and/or disinfectant treatment which may be effected by treating the mat after it is molded or adding the treatment compounds to the material from which the mat is molded.