A matrix for repairing and regenerating an articular cartilage and a method for preparing the matrix. The material of the matrix is a decalcified cancellous-cortical bone, and on the matrix a plurality of small holes (2) are provided to pass through the decalcified cancellous bone (3) and the decalcified cortical bone (1). The small holes (2) are arranged perpendicularly to the horizontal surface of the decalcified cortical bone (1). The diameter of the small holes (2) is 0.1-1mm, the number of the small holes (2) is more than two, and the distance between the centers of the adjacent small holes is 0.5-5mm. The method for preparing the matrix includes the followed steps: selecting a dual-layer bone comprising cortical bone and cancellous bone dipping the bone into a decalcifying liquid to decalcify the bone monitoring the progress of the decalcification by use of an atomic absorption spectrophotometer detecting the decalcified bone by use of the X-Ray and CT to ensure the bone has been decalcified completely, and obtaining a decalcified cancellous-cortical bone drilling holes with diameter of 0.1-1mm on the horizontal surface of the decalcified cortical bone (1) by use of a laser cutting the decalcified cancellous-cortical bone into certain size and specification, and storing them frozen. The present invention utilizes natural collagen matrix material which prevents the immunological rejection by users, facilitates the adsorption of cells, and has a good biocompatibility and a great mechanical strength.Linvention porte sur une matrice pour la réparation et la régénération dun cartilage articulaire et sur un procédé de préparation de la matrice. Le matériau de la matrice est un os spongieux-cortical décalcifié, et, sur la matrice, plusieurs petits trous (2) sont pourvus pour traverser los spongieux décalcifié (3) et los cortical décalcifié (1). Les petits trous (2) sont agencés perpendiculairement à la surface horizontale de los cortical décalcifié (1). Le diamètre d
