BEY RUSSELL F.,SIMONSON RANDY R.,SIRIGIREDDY KAMESH REDDY,HAUSE BENJAMIN MATTHEW
申请号：
NZ62828213
公开号：
NZ628282A
申请日:
2013.02.14
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is an immunological composition comprising: a) NSP4, VP4 and VP6 polypeptides； and b) a pharmaceutically or veterinary acceptable vehicle, diluent or excipient. Further disclosed is a method for producing the above composition comprising the steps of: (a) obtaining a biological sample from a non-human animal known or suspected of being infected with one or more types of rotavirus； (b) if infection status is not known, determining whether the animal is infected with rotavirus； (c) harvesting RNA from a biological sample previously obtained from the animal infected with the one or more type of rotavirus； (d) performing reverse transcriptase PCR using primers complementary to NSP4, VP4 and VP6 genes； (e) inserting the PCR product from step (d) into a suitable non-human expression vector； (f) placing the vector produced in step (e) into a suitable non-human host expression system； (g) harvesting the rotaviral polypeptides； and (h) adding to the rotaviral polypeptides any additional vaccine components, thereby producing the vaccine composition.
